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Macromolecules, 33, 5730-5739. Table 5 shows the data of Mark-Houwink parameters determined in figure 4 from linear regressions with 2 values higher than 0.9797. Superior prebiotic and physicochemical properties of novel dextran from Weissella cibaria JAG8 for potential food applications. Saudi Pharm J. The human and pig skin samples were placed in the receiver chamber of the Transdermal diffusion cell system with the SC facing up and the donor chamber was fixed in place. ; Wood, P.J. Molecular weight, particle size and diffusion coefficient can be determined in many studies by Dynamic Light Scattering [20, 21, 22], Gel Permeation Chromatography [23], Analytical Ultracentrifuge [24, intrinsic viscosity [1, 7, 14], size exclusion chromatography / multiangle laser light scattering / differential viscometry, Flow Field-Flow Fractionation [25], and so on. Radiofrequency ablation of malignant liver tumors. As intrinsic viscosity of dextrans decreases with an increase in solution temperature; chain flexibility enhances with an increase in temperature (see Table 4) showing that the intrinsic viscosity of dextran (with different molecular weights Mw from 8.8 to 200kDa) decreases linearly with an temperature increase in solution, but change to high molecular weight to hyperbranched macromolecule. Web54 we estimated that the hydrodynamic radii of 3-5 kDa dextran, 20 kDa dextran, and 70 kDa dextran are 2 55 nm, 5 nm, and 6 nm, respectively. Durand, A. ; Picout, D.R. Interaction Parameter (); Expansion Factor (); Steric Hindrance Factor (); and Shielding Function F(); for the System PEAOrganic Solvents by Intrinsic Viscosity Measurements. Biophysical Journal, 87, 4259-4270. Increased TEWL values are evidence of micropores formation. Catiker, E.; Guner, A. Synthesis of dextran by dextransucrase treated, sucrose-supplemented skimmed milk revealed the promising potential of dextran as a food additive. Proc. Progr. Just in case anyone wants to use a different molecular weight to estimate the hydrodynamic size: the calculator is integrated into the classic Zeta Working with the molecular weights given by the manufacturer and compare them with the molecular weights, calculated from the Mark-Houwink parameters in this work; can be seen that the %RE obtained, see Table 6. Lee WR, Shen SC, Sun CK, Aljuffali IA, Suen SY, Lin YK, Wang JJ, Fang JY. GPC Symp. FITC-derivatives have been used for a variety of biological applications for more than 40 yeras. It has been found that, when applied to water treatment, it can effectively act on refractory organic matter in wastewater through thermal decomposition and water molecular action to produce free The micropore formed at the SC through to the epidermis, enabling drug delivery. IV. The explanation for the anomalous values of a and kM-H is because the mixture solute-solvent is highly compatible due a great compaction of biopolymer, from undisturbed state in the absence of interactions, where free energy of mixing less than zero, and emphasize the nature non-linear or hyperbranched of dextran for Mw high to 200kDa. WebThe hydrodynamic radius ( Rh) determined by our Fluidity instruments is the Stokes radius, or the size of a hard spherical particle that diffuses at the same rate as the protein (s) detected. 4, 10, and 20K) was measured using the Transdermal diffusion cell system. what will you do to keep amazon safe answers; amarsi assisted living; peter clarke, emilia clarke; personality characteristics of kiran mazumdar shaw; karina mitchell age; mirela romanian orphan died; dsa presidential endorsement; dextran hydrodynamic radius. Pamis, R.; Hernndez Cifre, J. G.; Lpez Martnez, M.C. 2018;1:10915. TdB Labs produce a wide range of FITC-derivatives (490 nm/520 nm) with a mean molecular weight ranging from 4 kDa to 2000 kDa. 4K, b M.W. ; Talsma, H.; Hennink, W.E. Journal of Controlled Release, 119, 5-24. These studies were again focused on investigating the hydrodynamic properties of the polymer molecules, their hydrodynamic radius in relation to their radius of gyration and the coherence of the simulation results with polymer theory. The Principle of the Suspended Level: Applications to the Measurement of Viscosity and Other Properties of Liquids. Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. Figure4 shows the histological analysis of micropores in human cadaver skin. Carbohydrate Polymers 95 360-365. dextran hydrodynamic radius. Ghimici, Luminita; Nichifora, Marieta; Eich, Andreas; Wolf, Bernhard A. (2007). Structural characterization of a new dextran with a low degree of branching produced by Leuconostoc mesenteroides FT045B dextransucrase. (2001). ; Veldhuis, T.F.J. A long-chain polymer molecule in solution takes on a somewhat kinked or curled shape, intermediate between a tightly curled mass (coil) and a rigid linear configuration. The 70-kDa FITC dextran reached the jejunum after 15 min and passed the entire small intestine within 1 h after its administration, demonstrating that 4-kDa FITC Dextran Triple Detector Application Note 1, Viscotek GmbH, USA. This site needs JavaScript to work properly. There is a deviation from linearity after 580kDa [69, 70]. In this work, we evaluate the maximum radius of macromolecules able to enter into HeLa cells following a plasma treatment using a size exclusion approach with dextran molecules. Kano S, Todo H, Furui K, Kenichi S, Tokudome Y, Hashimoto F, Kojima H, Sugibayashi K. Comparison of several reconstructed cultured human skin models by microscopic observation: their usefulness as an alternative membrane for skin in drug permeation experiments. The solutions were prepared in bidestilated water at 1, 0.75, 0.5, 0.25 % wt. This study demonstrated that hydrodynamic volume and radius of gyration of a dextran sample with M w < 20 kDa and its linear counterpart with equal M w are almost Our portfolio includes for example derivatives of dextran, polysucrose, inulin and more. The dextran used is from Leuconostoc mesenteroides B-512F which is essentially a linear -(1-6)-linked glucose chain with however a low percentage (2-5%) of -(1-3) branches distributed along the chain4. ; Bos, G.W. Fractional thermolysis by bipolar radiofrequency facilitates cutaneous delivery of peptide and siRNA with minor loss of barrier function. Epub 2017 Jun 1. Coviello, T.; Matricardi, P.; Marianecci, C.; Alhaique, F. (2007). One day before the experiment, the skin was stabilized in the skin culture medium. (1990). Journal of Pharmaceutical and Biomedical Analysis, 41, 1260-1267. Article Alternatively, the value of qM-H can be calculated using numerical methods and other-average molecular weights (Mn, Mw, Mz) according to where c and d are empirical polynomial functions of the exponent a. The human and pig skin permeability of FITC-dextran (M.W. WebFluorescein isothiocyanatedextran average mol wt 60,000-76,000; CAS Number: 60842-46-8; Synonyms: FITCDextran; find Sigma-Aldrich-FD70S MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich Named after George Gabriel Stokes, it is closely related to solute mobility, factoring in not only size but also solvent effects.A smaller ion with stronger hydration, for example, may have a greater Stokes radius than a larger ion with Carbohydrate Research, 340, 811-826. 2010;4(1):117. Deng H, Konopka CJ, Prabhu S, Sarkar S, Medina NG, Fayyaz M, Arogundade OH, Vidana Gamage HE, Shahoei SH, Nall D, Youn Y, Dobrucka IT, Audu CO, Joshi A, Melvin WJ, Gallagher KA, Selvin PR, Nelson ER, Dobrucki LW, Swanson KS, Smith AM. The Dextrans intrinsic viscosity is determined as a function of the molecular weight in water and NaOH 0.5 M see reference [1]. The Mark-Houwink parameters indicate the dependence with temperature (T) in the range from 20 to 50C, ie with increasing T a increases and kM-H decreases. The human skins were from the back or thigh of a 24-year-old woman. Advances in Carbohydrate Analysis, 1, 63-144. The above data are not useful because it can not be compared with those obtained in this work. Dextran theta temperature between 43.14 and 44.44C for all the range of molecular weights explains the deviation at 45 and 50C [36, 37]. Walkenhorst, S.; Olivier, R. (2003). Universal size-independent quantities for the conformational characterization of rigid and flexible macromolecules. Similar results are obtained in references [35, 36, 37], using a system of dextran / ethylene glycol, and dextran/dimethylsulfoxide, respectively. The increased TEWL values which were proportional to the number of microspores indicate SC disruption and formation of microchannels in the skin. ; Rani, K.Y. Therefore, the trend of "a" and kM-H decreases with T, where the presence of ethylene glycol or dimethylsulfoxide strongly changes the interactions of this solvent with the macromolecule. Guner, Ali (1999). In this sense, the largest deviations were obtained for the highest molecular weight (580-2000kDa with values from 0.8467 to 0.7346). In vitro studies with human cadaver skin is a viable, ethical, and economical alternative for in vivo animal studies. Normally, the skin loses very little water loss whereas damaged skin loses more [22]. Trop J Pharm Res. ; Fran, H.P. Drug Deliv. Carbohydrate Polymers, 79, 380-390. Soc., 21, 1, 78-86. 2012;101(8):28619. The M-H equation is empirical and valid for monodisperse polymers, and it is applied to biomacromolecules (complex, hyperbranched, crosslink), taking the precaution of comparing the value of Mv with the polydispersity index (qM-H). The fast increasing of these polyglucosans for medical, industrial and research purposes motivated a survey of the types obtainable. Huang S, Huang G. Preparation and drug delivery of dextran-drug complex. Drug Deliv. The hydrodynamic radius R h, defined as the radius of a hard sphere that diffuses at the same rate as the solute, takes these effects into account. Appl Biochem Biotechnol. Please enable it to take advantage of the complete set of features! a Microscopy images of micropore and b quantified data using ImageJ after 0 (immediately), 4, 8, and 24h according to the number of times microproration was done (n=150, *: p<0.05). In the future, we plan to investigate the possibility of enhancing the delivery of other macromolecular drugs such as peptides and siRNAs using the RF-based microporation device. WebThomas Gutberlet works at Jlich Centre for Neutron Science (JCNS-2), Forsch and is well known for Lipoteichoic Acid, Neutron Reflectometry and Neutron Reflectivity. Characterization of high molecular weight dextran produced by Weissella cibaria CMGDEX3. Mark-Houwink values confirm that for these conditions dextrans behave as rigid spheres with a tendency at compaction as temperature increases. Chem. HHS Vulnerability Disclosure, Help Use of the single point method is correct because it is based in the Huggins method (HM); owing the HM requiring many viscosity and density measurements (at least four) for each Mw, but Solomon Ciuta method with a single measure is sufficient. From Table 1, It is worth noting that the value of 2 is above 0.95, being an acceptable value for viscosimetric measurement. Terms and Conditions, Chem. Hi Khanh, Alan is taking a little time off of Research Gate at the moment, but here is some information in reply to your question. Strategies to improve the potential of transdermal devices by enhancing the skin permeation of therapeutic entities. The skin culture medium was purchased from Biopredic International (Saint-Grgoire, France). Intrinsic viscosities of polyelectrolytes in the absence and in the presence of extra salt: Consequences of the stepwise conversion of dextran into a polycation. Cytotoxicity studies on human cervical cancer (HeLa) cell line showed non-toxic and biocompatible nature of dextran. Increased epidermal laser fluence through simultaneous ultrasonic microporation. Walkenhorst, S.; Olivier, R. (2002). Amersham Biosciences, AA Edition, www.amershambiosciences.com. Journal Non-Newtonian Fluid Mech., 97, 295-301. WebLabeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the Because of the recent animal protection rules which have restricted animal experiments [19], we used human cadaver skin as an alternative to animals. Fixable: Fixable via Lysine. Hydrodynamic cavitation (HC), a common phenomenon that can produce local excessive temperature, high pressure and large amount of energy. WebAccording to the above theoretical analysis for the micro-roughness contact model, the relationship between the hydrodynamic pressure (HP) and the contact pressure (CP) is shown in Fig. Int J Pharm Investig. Biological skin substitutes for wound cover and closure. Chapter 2.1.4 Suspended level viscometers for transparent liquid, Ubbelohde Viscometer page 28-31. Lim SB, Tingirikari JMR, Seo JS, Li L, Shim S, Seo JH, Han NS. Hydrodynamic properties, such as the intrinsic viscosity, [], and equilibrium solution properties such as the hydrodynamic radius RH can be combined to construct dimensionless quantities that are universal since they are of being independent of the macromolecular particle size, while they depend more or less sensitively on its shape [51]. Carbohydrate Polymers, 36, 319-326. Molecular Weight Distributions of Water-Soluble Polymers by Flow Field-Flow Fractionation. Hydrodynamic Properties of Tragacanthin. Expert Opin Drug Deliv. The Solomon-Ciuta single-point method is used for calculating dextran intrinsic viscosity and the error relative is calculated, and %RE respect to Huggins method is >4.18% for 2000kDa, and >2.06% for 8.8kDa (Table 2). European. Dextran standards with sequence molecular weight from 8.8, 40, 71.9, 110, 200, 580 and 2000 kDa were utilized for the plot of calibration curve. WebHydrodyamic Radius, R H: In addition to the analytic size of a linear (C=1) chain, the end to end distance, R eted or R 0, and the structural size, the radius or gyration, R g, dynamic measurements yield a size called the hydrodynamic radius, R H. Consider a rod particle of length L=10R where R is the radius. Drug deliv. (1999). Unperturbed dimensions and the theta temperature of dextran in ethylene glycol solutions. J Cosmet Laser Ther. NCI CPTC Antibody Characterization Program. It is a measure of the width of molecular weight distributions (MWD) as well as probability of molecular weight distribution curve (GPC). Learn More. Would you like email updates of new search results? A lot of research has been devoted to dextrans modification in order to describe special characteristics relevant to specific applications such as surfactants [16], as visible marker [17], polysaccharides-based nanoparticles, covalent crosslinking [18], ionic crosslinking, polyelectrolyte complex, and the self-assembly of hydrophobically modified polysaccharides, and hydrophilic modified polysaccharides [19]. WebHydrodynamic radius = variable size of an analyte with specific MW based on dynamical properties. Bahary, W. S.; Hogan, M. P.; Jilani, M.; Aronson, M. P. (1995). In a good solvent, which shows a zero or negative heat of mixing with the polymer, the molecule is less-extended, and the intrinsic viscosity is high. (1937). The important role of thermodynamic interaction parameter in the determination of theta temperature, dextran/water system. Data File Dextran. Int J Food Sci Nutr. Gel permeation chromatography of dextrans in parenteral solutions: Calibration procedure development and method validation. The value of Mv is different from Mn, Mw, and Mz in a polydisperse polymer sample [38, 60, 61, 62]. Tirtaatmadja, V.; Dunstan, D. E.; Boger, D. V. (2001). Webprotein hydrodynamic radius was observed with increasing protein concentration (in the range 0.25-2 mM) in both the absence and presence of 8 M urea. Int. WebThe invention relates to novel fluorescent dyes with multiple negatively charged groups in their ionized form which are 9-aminoacridines or 1-aminopyrene shaving of one of the fol Typically, the hydrodynamic diameter of the micelles is about 100 nm and the critical micelle concentration is about 10 mg/L. A solvent at this temperature is called a theta solvent [53]. Santoro MM, Gaudino G. Cellular and molecular facets of keratinocyte reepithelization during. 2009;6(4):34354. "Dextrans in Aqueous Solution. Viswanath, D.S. ; Weisz, J. Carbohydrate Polymers, 39, 377-380. Challenges in analysis of high-molar mass dextrans: Comparison of HPSEC, AsFlFFF and DOSY NMR spectroscopy. Polyelectrolytes as secondary calibration standards for aqueous SEC. This phenomenon is observed in the case of hyperbranched biopolymers evidencing an increase of a with temperature. Springer Editions. Chromatographic characterization of polymers: Hyphenated and multidimensional techniques. ; Yevlampieva N.P. Provided by the Springer Nature SharedIt content-sharing initiative. The effectiveness of the RF microporation technique in transdermal drug delivery of macromolecules and hydrophilic agents such as peptides, hormones, and vaccines has been demonstrated extensively in previous studies [16,17,18]. Dextran molecular size and degree of branching as a function of sucrose concentration, pH, and temperature of reaction of. of different origins. This result could reflect the presence of some aggregated species within the lysozyme sample solution. The proportionality between []0 and M0.5 is obtained as a limiting law for the no draining regime. WebTry out PMC Labs and tell us what you think. CAS The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material.